Nine micropollutant substances have been identified as high priority substances to be reported in all case studies based on their environmental relevance, coverage of chemical and physical properties, elimination rates by various technologies and existence of an analytical method to identify them. Characterization factors for the toxicity model USEtox® have been developed for all these substances based on the EPISuite v4.1 software for physico-chemical properties, data from project partners (EAWAG) for aquatic toxicity properties and a literature review for human toxic properties.